Backes D W, Aboleneen H I, Simpson J A
Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064-3502, USA.
J Pharm Biomed Anal. 1998 Apr;16(8):1281-7. doi: 10.1016/s0731-7085(97)00140-4.
The delayed clearance of vancomycin results in accumulation of vancomycin crystalline degradation product, CDP-1, in the bodies of renally impaired patients. The 2 isomers, CDP-1-M (major) and CDP-1-m (minor), of CDP-1 are antibiotically inactive but cross-react with some immunoassays that use polyclonal antibodies resulting in falsely elevated results. A high performance liquid chromatographic (HPLC) method was developed to quantitate vancomycin and CDP-1 in the serum of renal patients. After solid phase extraction of 200 microliters serum, the separation of vancomycin, the 2 isomers of CDP-1 and the internal standard (cefazolin) was accomplished by gradient HPLC on a reversed phase C18 column with detection at 210 nm. Linearity was established from 1 to 25 and 25 to 100 micrograms ml-1 vancomycin and 1 to 25 micrograms ml-1 CDP-1. Coefficients of variation for vancomycin and CDP-1 were 3.3-8.6% (n = 10) and 2.8-5.2% (n = 8).
万古霉素清除延迟会导致万古霉素结晶降解产物CDP - 1在肾功能受损患者体内蓄积。CDP - 1的两种异构体，即CDP - 1 - M（主要）和CDP - 1 - m（次要），无抗菌活性，但会与一些使用多克隆抗体的免疫测定发生交叉反应，导致结果假性升高。开发了一种高效液相色谱（HPLC）方法来定量肾功能不全患者血清中的万古霉素和CDP - 1。在对200微升血清进行固相萃取后，通过在反相C18柱上进行梯度HPLC分离万古霉素、CDP - 1的两种异构体和内标（头孢唑林），检测波长为210 nm。万古霉素在1至25微克/毫升和25至100微克/毫升以及CDP - 1在1至25微克/毫升范围内建立了线性关系。万古霉素和CDP - 1的变异系数分别为3.3 - 8.6%（n = 10）和2.8 - 5.2%（n = 8）。
