Stafford J E, Lees S, Watson D
J Clin Pathol. 1976 Jul;29(7):642-7. doi: 10.1136/jcp.29.7.642.
Two radioimmunassays for the determination of serum triiodothyronine (T3) were developed. The assay of T3 in unextracted serum had several advantages over the assay on extracted serum and was chosen for the routine determination of T3 in serum from 117 patients requiring assessment of their thyroid status. In 53 subjects considered retrospectively not to have thyroid dysfunction nor to have been on steroid contraceptives or therapy, the pooled mean serum T3 concentration was 1-92 (actual range 0-88-2-62) nmol/l. A significant inverse relationship was observed between the serum T3 level and the age of the subject. Serum total T3 levels discriminate clearly between hypo-,eu- and hyperthyroid patients and provide a rather more sensitive index of hyperthyroid function than total serum T4. In the face of normal serum T4 the T3 level was depressed in five patients with marked hypoproteinaemia and elevated in two patients taking heroin.
开发了两种用于测定血清三碘甲状腺原氨酸（T3）的放射免疫分析方法。与对提取血清进行的检测相比，未提取血清中T3的检测具有多个优点，因此被选用于对117例需要评估甲状腺状态的患者血清中的T3进行常规测定。在53例回顾性分析认为既无甲状腺功能障碍，也未使用甾体类避孕药或接受甾体类治疗的受试者中，血清T3浓度的合并均值为1.92（实际范围0.88 - 2.62）nmol/L。观察到血清T3水平与受试者年龄之间存在显著的负相关关系。血清总T3水平能清晰地区分甲状腺功能减退、正常和亢进患者，并且与血清总T4相比，能提供一个对甲状腺功能亢进更为敏感的指标。在血清T4正常的情况下，5例明显低蛋白血症患者的T3水平降低，2例服用海洛因的患者T3水平升高。
