Premachandra B N
J Nucl Med. 1976 May;17(5):411-6.
A simple, accurate, and rapid procedure for radioimmunoassay (RIA) of triiodothyronine (T3) is described. Serum is denatured by sodium trichloroacetate and the T3 released is allowed to react with 125I-T3-labeled T3 anti-serum. The displaced 125I-T3 is rapidly taken up by an anionic-resin sponge, and this uptake is linearly related to serum T3 concentration. The entire assay procedure was completed in the same tube in about 1 hr. The T3 antibody was specific, and T4-to-T3 conversion in the assay did not occur. Recovery of exogenously added T3 was virtually complete. The mean T3 concentration in 92 euthyroid subjects (142 ng/100 ml) is comparable to the values described in a number of radioimmunoassays for T3.
本文描述了一种用于三碘甲状腺原氨酸（T3）放射免疫分析（RIA）的简单、准确且快速的方法。血清用三氯乙酸钠变性，释放出的T3与125I-T3标记的T3抗血清反应。被置换出的125I-T3迅速被阴离子树脂海绵吸收，这种吸收与血清T3浓度呈线性关系。整个分析过程在同一试管中约1小时内完成。T3抗体具有特异性，分析过程中未发生T4向T3的转化。外源性添加T3的回收率几乎是完全的。92名甲状腺功能正常受试者的平均T3浓度（142 ng/100 ml）与许多T3放射免疫分析中描述的值相当。
