胃液中葡萄球菌肠毒素的检测
Detection of staphylococcal enterotoxin in gastric juice.
作者信息
Dickie N, Park C E, Robern H
出版信息
J Clin Pathol. 1976 Sep;29(9):833-5. doi: 10.1136/jcp.29.9.833.
The gastric juice of a patient showing symptoms of staphylococcal food poisoning was examined by a radioimmunoassay for the presence of enterotoxins. Assays gave markedly higher results at 35 degrees than at 5 degrees. The source for this discrepancy was attributed to interference due to trypsin activity on the basis of (1) the demonstration of hydrolysis of p-toluenesulfonyl-L-arginine methyl ester by the specimen, (2) inhibition of this activity by trypsin inhibitor from lima bean, and (3) lowered values produced for enterotoxins in gastric juice when the inhibitor was included in the assay system.
通过放射免疫分析法对一名出现葡萄球菌食物中毒症状患者的胃液进行检测，以确定其中是否存在肠毒素。检测结果显示，在35摄氏度时的结果明显高于5摄氏度时的结果。这种差异的来源归因于胰蛋白酶活性的干扰，依据如下：（1）标本对甲苯磺酰-L-精氨酸甲酯的水解作用得到证实；（2）利马豆中的胰蛋白酶抑制剂可抑制这种活性；（3）当在检测系统中加入该抑制剂时，胃液中肠毒素的检测值降低。
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