Armstrong J A, Bieker J J, Emerson B M
Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Cell. 1998 Oct 2;95(1):93-104. doi: 10.1016/s0092-8674(00)81785-7.
Erythroid Krüppel-like factor (EKLF) is necessary for stage-specific expression of the human beta-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active beta-globin promoter on chromatin templates in vitro. E-RC1 contains BRG1, BAF170, BAF155, and INI1 (BAF47) homologs of yeast SWI/SNF subunits, as well as a subunit unique to higher eukaryotes, BAF57, which is critical for chromatin remodeling and transcription with EKLF. E-RC1 displays functional selectivity toward transcription factors, since it cannot activate expression of chromatin-assembled HIV-1 templates with the E box-binding protein TFE-3. Thus, a member of the SWI/SNF family acts directly in transcriptional activation and may regulate subsets of genes by selectively interacting with specific DNA-binding proteins.
红系Krüppel样因子（EKLF）对于人类β-珠蛋白基因的阶段特异性表达是必需的。我们发现，EKLF需要一种与SWI/SNF相关的染色质重塑复合物，即EKLF共激活因子-重塑复合物1（E-RC1），以便在体外染色质模板上生成对DNase I敏感的、具有转录活性的β-珠蛋白启动子。E-RC1包含酵母SWI/SNF亚基的BRG1、BAF170、BAF155和INI1（BAF47）同源物，以及高等真核生物特有的一个亚基BAF57，它对于与EKLF一起进行染色质重塑和转录至关重要。E-RC1对转录因子表现出功能选择性，因为它不能通过E盒结合蛋白TFE-3激活染色质组装的HIV-1模板的表达。因此，SWI/SNF家族的一个成员直接参与转录激活，并且可能通过与特定的DNA结合蛋白选择性相互作用来调节基因子集。
