Phelan M L, Sif S, Narlikar G J, Kingston R E
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA.
Mol Cell. 1999 Feb;3(2):247-53. doi: 10.1016/s1097-2765(00)80315-9.
Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.
SWI/SNF家族的蛋白质复合物以ATP依赖的方式重塑核小体结构。每个复合物包含8到15个亚基，其中几个在酵母、果蝇和人类之间高度保守。我们使用一组保守亚基重建了一个ATP依赖的染色质重塑复合物。出乎意料的是，人类SWI/SNF复合物的ATP酶亚基BRG1和hBRM作为纯化蛋白都能够重塑单核小体和核小体阵列。将INI1、BAF155和BAF170添加到BRG1中可将重塑活性提高到与整个hSWI/SNF复合物相当的水平。这些数据定义了hSWI/SNF复合物的功能核心。
