Kammerer M, Pouliquen H, Pinault L, Loyau M
Centre d'Informations Toxicologiques Veterinaires de Nantes, Ecole Nationale Veterinaire de Nantes, France.
Vet Hum Toxicol. 1998 Oct;40(5):273-5.
Accidental ingestion of anticoagulant rodenticide bait by poultry rarely leads to clinical signs of poisoning, but represents a risk for the consumer because of potential residues in the laid eggs. An assay was conducted for a better risk assessment. Three groups of laying hens were given a single oral dose of 10, 30 or 90 mg warfarin/kg BW. Eggs were collected for 14 d, and warfarin was analyzed by reversed-phase high-performance liquid chromatography. Warfarin was present in the white for 3 to 4 d following anticoagulant ingestion, while concentration increased in the yolk until the 5th or 6th d, and then gradually decreased. At the end of experimentation, warfarin was still detected at below 100 ng/g in the yolk of eggs in each group.
家禽意外摄入抗凝血灭鼠剂诱饵很少会导致中毒的临床症状，但由于所产鸡蛋中可能存在残留，这对消费者构成了风险。为了进行更好的风险评估，开展了一项检测。三组产蛋母鸡分别经口单次给予10、30或90毫克华法林/千克体重。收集鸡蛋14天，并通过反相高效液相色谱法分析华法林。摄入抗凝剂后，蛋清中可检测到华法林3至4天，而蛋黄中的浓度在第5或6天之前持续增加，然后逐渐下降。实验结束时，每组鸡蛋的蛋黄中仍能检测到低于100纳克/克的华法林。
