采用反相高效液相色谱法测定鸡蛋蛋黄和蛋清中的华法林。
Determination of warfarin in the yolk and the white of hens' eggs by reversed-phase high-performance liquid chromatography.
作者信息
Pouliquen H, Fauconnet V, Morvan M L, Pinault L
机构信息
Ecole Nationale Vétérinaire de Nantes, Laboratoire de Pharmacie et Toxicologie, Atlanpôle-La Chantrerie, France.
出版信息
J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):143-8. doi: 10.1016/s0378-4347(97)00382-4.
A procedure for the determination of warfarin, an anticoagulant rodenticide, in the white and the yolk of hens' eggs, using reversed-phase high-performance liquid chromatography is described. Liquid chromatography was performed on an octadecylsilane cartridge using methanol and ammonium acetate triethylamine buffer as the mobile phase, with UV detection at 281 nm. Samples (5 g) were analysed after liquid-phase extraction using a mixture of acetone and diethyl ether. Linearity, precision and accuracy of the method were determined in the range of 0.5-8.0 microg. Limits of quantitation for warfarin in the white and the yolk were 0.020 and 0.015 microg/g, respectively. Mean recoveries of warfarin from spiked white and yolk samples were 84.6 and 87.4%, respectively. The analytical method was applied to a fourteen-day experimental study conducted in laying hens that had been orally dosed with warfarin.
本文描述了一种使用反相高效液相色谱法测定鸡蛋蛋白和蛋黄中抗凝灭鼠剂华法林的方法。液相色谱分析在十八烷基硅烷柱上进行，以甲醇和醋酸铵 - 三乙胺缓冲液为流动相，于281 nm处进行紫外检测。使用丙酮和乙醚的混合物进行液 - 液萃取后，对5 g样品进行分析。该方法在0.5 - 8.0 μg范围内测定了线性、精密度和准确度。蛋白和蛋黄中华法林的定量限分别为0.020和0.015 μg/g。加标蛋白和蛋黄样品中华法林的平均回收率分别为84.6%和87.4%。该分析方法应用于一项对华法林口服给药的蛋鸡进行的为期14天的实验研究。
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