van der Wilk F, Verbeek M, Dullemans A, van den Heuvel J
Department of Virology, DLO Research Institute for Plant Protection (IPO-DLO), Wageningen, The Netherlands.
Virus Genes. 1998;17(1):21-4. doi: 10.1023/a:1008044715899.
The sequence of the 20 N-terminal amino acids of the viral protein (VPg) which is covalently attached to the genomic RNA of the bean strain of Southern bean mosaic virus (SBMV-B) has been determined. The obtained VPg sequence mapped to position 327 to 346 of the SBMV-B ORF2 product, downstream of the putative protease domain and in front of the RNA-dependent RNA polymerase. Thus indicating that the sobemovirus genomic arrangement is similar to that of subgroup II luteoviruses. Comparison with other viral sequences revealed a high similarity with the sequence of the ORF2-product of the cowpea strain of SBMV (SBMV-C). No significant similarities were detected with amino acid sequences derived of other sobemoviruses or non-related viruses.
已确定共价连接到南方菜豆花叶病毒豆株系(SBMV-B)基因组RNA上的病毒蛋白(VPg)20个N端氨基酸的序列。所获得的VPg序列定位于SBMV-B ORF2产物的327至346位,在假定的蛋白酶结构域下游且在RNA依赖性RNA聚合酶之前。因此表明南方菜豆花叶病毒基因组排列与II亚组黄症病毒相似。与其他病毒序列比较显示与SBMV豇豆株系(SBMV-C)的ORF2产物序列高度相似。未检测到与其他南方菜豆花叶病毒或非相关病毒衍生的氨基酸序列有显著相似性。