Okotie-Eboh G, Gerguis J, Black H S
Veterans Affairs Medical Center, Houston, TX 77030, USA.
Photodermatol Photoimmunol Photomed. 1998 Jun-Aug;14(3-4):116-8. doi: 10.1111/j.1600-0781.1998.tb00024.x.
The influence of diets containing high (12%, w/w) and low (0.75%) levels of corn oil on hapten-specific antibody production to trinitrophenol-conjugated sheep red blood cells (TNP-SRBC) was examined in mice receiving 0, 3, 9, and 11 wk of UV radiation. Splenocytes from HRA HRII-c/+/Skh female hairless mice from the two dietary groups were incubated under a special atmosphere of low oxygen tension (7% O2, 10% CO2, and 83% N2) with TNP-SRBC to generate hapten-specific T-suppressor cells that, in turn, influence the number of direct plaque forming cells (PFC) in the Cunnigham-Szenberg plaque assay. Chronic UV irradiation reduced the number of direct PFC in both groups. After 11 wk of UV, the number of PFC in the high dietary fat group was significantly lower (P < 0.001) than that observed in the low fat group. These results suggest that dietary fat modulates UV-induced hapten-specific immunosuppression. Furthermore, the influence of dietary fat level, in this respect, was not realized until after 11 wk of UV, a time at which dietary fat has been shown to exert its influence on UV-carcinogenic expression.
在接受0、3、9和11周紫外线辐射的小鼠中，研究了含有高（12%，w/w）和低（0.75%）玉米油水平的饮食对针对三硝基苯酚偶联绵羊红细胞（TNP-SRBC）的半抗原特异性抗体产生的影响。将来自两个饮食组的HRA HRII-c/+/Skh雌性无毛小鼠的脾细胞在低氧张力（7% O2、10% CO2和83% N2）的特殊气氛下与TNP-SRBC一起孵育，以产生半抗原特异性T抑制细胞，进而影响Cunnigham-Szenberg空斑试验中直接空斑形成细胞（PFC）的数量。慢性紫外线照射减少了两组中的直接PFC数量。紫外线照射11周后，高膳食脂肪组中的PFC数量显著低于（P < 0.001）低脂组中观察到的数量。这些结果表明，膳食脂肪调节紫外线诱导的半抗原特异性免疫抑制。此外，在这方面，膳食脂肪水平的影响直到紫外线照射11周后才显现出来，此时已证明膳食脂肪会对紫外线致癌表达产生影响。
