一种使用环丙沙星消除细胞培养支原体的安全有效方法。

A safe and efficient method for elimination of cell culture mycoplasmas using ciprofloxacin.

作者信息

Schmitt K, Däubener W, Bitter-Suermann D, Hadding U

机构信息

Institute for Medical Microbiology, Johannes Gutenberg University, Mainz, F.R.G.

出版信息

J Immunol Methods. 1988 Apr 22;109(1):17-25. doi: 10.1016/0022-1759(88)90437-1.

DOI:10.1016/0022-1759(88)90437-1

PMID:3282011

Abstract

The antibacterial activity of ciprofloxacin, a 4-fluoroquinolone antibiotic, in the control of mycoplasma contamination in experimentally infected cell lines has been investigated. Seven mycoplasma species, including M. hyorhinis, M. gallisepticum, M. orale, M. salivarium, M. hominis, M. fermentans, and M. arginini, which had chronically infected the murine plasmocytoma line X63-Ag8 653, were eradicated with 10 micrograms/ml ciprofloxacin. Wild type laboratory infections of two human cell lines, HL-60 and U-937, were eliminated by 12 days of such treatment. Mycoplasma decontamination of cell cultures was monitored by the cultivation method 4 weeks after treatment. No side effects were seen in cell cultures and complex proliferation assays with cells of human and murine origin, using ciprofloxacin in doses up to 2.5 times the usual bactericidal concentration.

摘要

已对4-氟喹诺酮类抗生素环丙沙星在控制实验感染细胞系支原体污染方面的抗菌活性进行了研究。用10微克/毫升环丙沙星根除了长期感染鼠浆细胞瘤系X63-Ag8 653的7种支原体，包括猪鼻支原体、鸡毒支原体、口腔支原体、唾液支原体、人型支原体、发酵支原体和精氨酸支原体。通过12天的这种治疗消除了两个人类细胞系HL-60和U-937的野生型实验室感染。治疗4周后通过培养法监测细胞培养物的支原体清除情况。在细胞培养以及对人和鼠源细胞进行的复杂增殖试验中，使用高达通常杀菌浓度2.5倍剂量的环丙沙星未观察到副作用。