Medina V, Tian T, Wierzchos J, Falk B W
Dept de Producció Vegetal I Ciència Forestal de la Universitat de Lleida, Spain.
J Gen Virol. 1998 Oct;79 ( Pt 10):2325-9. doi: 10.1099/0022-1317-79-10-2325.
Nicotiana benthamiana mesophyll protoplasts, either mock-inoculated or inoculated using in vitro transcripts derived from lettuce infectious yellows virus (LIYV) RNA 1- and/or RNA 2-cloned cDNAs were analysed by transmission electron microscopy (TEM) and, in some cases, also by immunogold labelling. TEM revealed the main cytopathological effects of LIYV infections in N. benthamiana protoplasts infected with RNAs 1 and 2: (a) typical closterovirus-induced (beet yellows virus-type) accumulations of vesiculated cytoplasmic membranes as inclusion bodies, sometimes with associated virions; (b) scattered aggregations of virions within the cytoplasm; and (c) electron-dense plasmalemma deposits. These were not seen in mock-inoculated protoplasts. Protoplasts inoculated only with LIYV RNA 1 contained vesiculated cytoplasmic inclusion bodies, but not virions or plasmalemma deposits. Thus, infection by only LIYV RNA 1 is sufficient to induce characteristic closterovirus vesiculated cytoplasmic inclusion bodies. However, both LIYV RNAs 1 and 2 are needed for production of virions and plasmalemma deposits.
使用来自生菜传染性黄化病毒（LIYV）RNA 1和/或RNA 2克隆cDNA的体外转录本对接种或未接种的本氏烟草叶肉原生质体进行了透射电子显微镜（TEM）分析，在某些情况下还进行了免疫金标记。TEM揭示了感染RNA 1和2的本氏烟草原生质体中LIYV感染的主要细胞病理学效应：（a）典型的长线形病毒诱导的（甜菜黄化病毒型）泡状细胞质膜聚集体作为包涵体，有时伴有相关病毒粒子；（b）细胞质内病毒粒子的散在聚集；以及（c）电子致密的质膜沉积物。在未接种的原生质体中未观察到这些现象。仅接种LIYV RNA 1的原生质体含有泡状细胞质包涵体，但没有病毒粒子或质膜沉积物。因此，仅LIYV RNA 1感染就足以诱导特征性的长线形病毒泡状细胞质包涵体。然而，产生病毒粒子和质膜沉积物需要LIYV RNA 1和2。
