Tarazi F I, Tomasini E C, Baldessarini R J
Mailman Research Center, McLean Division of Massachusetts General Hospital, Harvard Medical School Belmont 02178, USA.
Neurosci Lett. 1998 Sep 18;254(1):21-4. doi: 10.1016/s0304-3940(98)00644-2.
Density of dopamine transporter (DAT) and serotonin transporter (5-HTT) membrane proteins in the caudate-putamen (CPu) and nucleus accumbens (NAc) of rat brain was assessed at seven ages at postnatal days (PD) 7-60, by in vitro quantitative autoradiography. Binding of [3H]GBR-12935 (to DAT) and [3H]paroxetine (to 5-HTT) increased steadily and very similarly, from low levels at PD-7 to maximal levels, to 6-7-fold higher density at PD-60 in both regions. These findings indicate that DAT and 5-HTT follow a synchronized course of development in rat CPu and NAc. In contrast to reported elimination of excessive receptors in CPu and NAc during maturation, there was no evidence of pruning of DAT or 5-HTT in these regions of rat forebrain.
通过体外定量放射自显影技术，在出生后第7天至60天的7个年龄段，评估大鼠脑尾状核-壳核（CPu）和伏隔核（NAc）中多巴胺转运体（DAT）和5-羟色胺转运体（5-HTT）膜蛋白的密度。[3H]GBR-12935（与DAT结合）和[3H]帕罗西汀（与5-HTT结合）的结合从出生后第7天的低水平稳步且非常相似地增加至最高水平，在两个区域中出生后第60天的密度均比出生后第7天高6至7倍。这些发现表明，在大鼠CPu和NAc中，DAT和5-HTT遵循同步的发育过程。与报道的成熟过程中CPu和NAc中过量受体的消除相反，在大鼠前脑的这些区域中没有证据表明DAT或5-HTT存在修剪现象。
