Ono K, Yamamoto K, Niwa A
Section of Food Microbiology, Saitama Institute of Public Health.
Nihon Saikingaku Zasshi. 1998 Aug;53(3):519-29. doi: 10.3412/jsb.53.519.
Campylobacter jejuni were isolated from 7 epidemic outbreaks (121 isolates), 15 patients with gastroenteritis, chicken meats (47 isolates) and chicken cecal contents (70 isolates). The isolates and one standard strain of C. jejuni JCM2013 were analysed by randomly amplified polymorphic DNA method (RAPD). Total of 254 C. jejuni isolates were divided 68 different RAPD types which included strains that did not to divided by Lior's or Penner's serotyping system. To compare the similarities of RAPD patterns among the isolates, the amplification patterns of DNA were estimated by means of the Dice coefficient, and clustering of strains was based on the unweighted average pair group method (UPGMA) to facilitate the plotting of a dendrogram. It suggests that amplification band patterns of human isolates were different from those of chicken ones. Thus additional information given from RAPD profiles serves for epidemiological investigation and RAPD analysis is recommended as rapid and effective typing method.
空肠弯曲菌从7次疫情爆发（121株分离株）、15例肠胃炎患者、鸡肉（47株分离株）和鸡盲肠内容物（70株分离株）中分离得到。采用随机扩增多态性DNA方法（RAPD）对分离株和一株空肠弯曲菌标准菌株JCM2013进行分析。总共254株空肠弯曲菌分离株被分为68种不同的RAPD类型，其中包括未被Lior或Penner血清分型系统区分的菌株。为了比较分离株之间RAPD图谱的相似性，通过Dice系数估计DNA的扩增图谱，并基于非加权平均配对组方法（UPGMA）对菌株进行聚类，以便绘制树形图。结果表明，人类分离株的扩增条带模式与鸡源分离株不同。因此，RAPD图谱提供的额外信息有助于流行病学调查，推荐将RAPD分析作为一种快速有效的分型方法。
