Thorell J I, Holmström B
J Endocrinol. 1976 Sep;70(3):335-44. doi: 10.1677/joe.0.0700335.
Antisera were produced in rabbits against highly purified preparations of human LH (2000 or 10,000 i.u./mg), human FSH (5500 i.u./mg), and human TSH (7-5 i.u./mg). Most rabbits produced antisera of high titre and high avidity. Cross-reactions were minimal between human TSH and human chorionic gonadotrophin (HCG) and between human FSH and HCG but marked between human LH and HCG. TSH and FSH also showed a constant but relatively weak cross-reaction. LH cross-reacted with FSH to a higher degree than did HCG. The avidity of the antisera was high. It was concluded that much of the lack of specificity recorded for glycoprotein antisera are effects of impure immunogens. Some of the true cross-reactions are probably explained by shared antigenic determinants of the beta-subunits. Unadsorbed antisera could be used for assay of FSH and TSH in plasma from pregnant women.
用高度纯化的人促黄体生成素（2000或10,000国际单位/毫克）、人促卵泡生成素（5500国际单位/毫克）和人促甲状腺激素（7 - 5国际单位/毫克）制剂在兔体内制备抗血清。大多数兔子产生了高滴度和高亲和力的抗血清。人促甲状腺激素与人绒毛膜促性腺激素（HCG）之间以及人促卵泡生成素与HCG之间交叉反应极小，但人促黄体生成素与HCG之间交叉反应明显。促甲状腺激素和促卵泡生成素也表现出持续但相对较弱的交叉反应。促黄体生成素与促卵泡生成素的交叉反应程度高于HCG。抗血清的亲和力较高。得出的结论是，糖蛋白抗血清所记录的许多特异性缺乏是不纯免疫原的影响。一些真正的交叉反应可能是由β亚基的共同抗原决定簇解释的。未吸附的抗血清可用于检测孕妇血浆中的促卵泡生成素和促甲状腺激素。
