Hardy J, Bertone A L, Muir W W
Department of Veterinary Clinical Sciences, The Ohio State University, Columbus 43210, USA.
Am J Vet Res. 1998 Oct;59(10):1307-16.
To determine oxygen metabolism, permeability, and blood flow in isolated joints in response to interleukin 1beta (IL-1beta) and contribution of innervation.
One metacarpophalangeal (MCP) joint of 24 adult horses.
The MCP joint was isolated for 6 hours in a pump-perfused, auto-oxygenated, innervated or denervated preparation. Isolated joints were assigned to the following 4 groups: control, control-denervated, inflamed, and inflamed-denervated, and inflammation was induced by intra-articular injection of IL-1beta. Circuit arterial and venous pressures, flows, and blood gas tensions, synovial fluid production, and intra-articular pressure were measured. Total vascular resistance; oxygen delivery, consumption, and extraction ratio (ER); and permeability surface area product were calculated. Synovial membrane blood flow was determined at 0, 60, and 330 minutes. Synovial membrane wet-to-dry ratio was obtained, and permeability to macromolecules was determined by intra-articular injection of Evans blue albumin and fluorescein isothiocyanate-conjugated dextran.
Oxygen delivery and synovial membrane blood flow progressively increased but were not different among groups. Oxygen consumption and ER significantly increased in inflamed joints, as did intraarticular pressure and synovial fluid production. Inflamed joints had greater wet-to-dry ratio. Albumin permeability significantly increased in the villous synovial membrane of the inflamed groups, and dextran permeability was increased in the innervated groups, with a trend toward increased permeability in inflamed groups.
Inflammation significantly increased oxygen demand, which was initially met by increased ER. Permeability to small molecules was increased with inflammation; innervation increased permeability to large molecules. Use of an isolated joint model enabled documentation of the physiologic responses of the joint to acute inflammation.
确定离体关节对白细胞介素1β（IL-1β）的氧代谢、通透性和血流情况以及神经支配的作用。
24匹成年马的一个掌指关节（MCP）。
将MCP关节在泵灌注、自动充氧、有神经支配或去神经支配的制备条件下分离6小时。离体关节被分为以下4组：对照组、去神经支配对照组、炎症组和去神经支配炎症组，通过关节内注射IL-1β诱导炎症。测量循环动脉和静脉压力、血流、血气张力、滑液生成和关节内压力。计算总血管阻力、氧输送、消耗和提取率（ER）以及通透表面积乘积。在0、60和330分钟时测定滑膜血流。获得滑膜湿干比，并通过关节内注射伊文思蓝白蛋白和异硫氰酸荧光素偶联葡聚糖测定对大分子的通透性。
氧输送和滑膜血流逐渐增加，但各组间无差异。炎症关节中的氧消耗和ER显著增加，关节内压力和滑液生成也增加。炎症关节的湿干比更大。炎症组绒毛滑膜中的白蛋白通透性显著增加，去神经支配组中葡聚糖通透性增加，炎症组有通透性增加的趋势。
炎症显著增加氧需求，最初通过增加ER来满足。炎症使小分子通透性增加；神经支配使大分子通透性增加。使用离体关节模型能够记录关节对急性炎症的生理反应。
