Motlik J, Fulka J
J Exp Zool. 1976 Nov;198(2):155-62. doi: 10.1002/jez.1401980205.
The breakdown of the pig germinal vesicle (GV) was studied in follicular oocytes matured in vivo and in vitro. For better characterization, the whole process was divided into four well-defined stages, based on the chromatin changes, and on nucleolus and nuclear membrane disappearance. In the intact germinal vesicle (GV I) nuclear membrane and nucleolus are clearly visible and chromatin forms a ring or horseshoe around the nucleolus. In the GV II a fre orceinpositive structures (chromocenters) on the nuclear membrane can be detected. For the GV III slightly stained chromatin clumps, localized especially around the nucleolus, and the beginning of strand formation are typical. In the last stage - GV IV - the nuclear membrane is less distinct and the nucleolus disappears completely. Chromatin is seen as an irregular network or as individual bivalents. According to these criteria GV breakdown in vivo was completed in most oocytes between 20 to 24 hours after HCG injection. In culture a similar stage of development was reached between 16 to 20 hours. This difference in the progress of nuclear changes appeared at the beginning of nuclear maturation and remained unchanged throughout the whole period studied.
对体内和体外成熟的卵泡卵母细胞中猪生发泡（GV）的破裂进行了研究。为了更好地表征，根据染色质变化、核仁和核膜消失情况，将整个过程分为四个明确的阶段。在完整的生发泡（GV I）中，核膜和核仁清晰可见，染色质在核仁周围形成环或马蹄形。在GV II中，可以检测到核膜上的游离或orcein阳性结构（染色中心）。对于GV III，典型的是染色质团块轻度染色，尤其位于核仁周围，并且开始形成链。在最后阶段 - GV IV - 核膜不太明显，核仁完全消失。染色质表现为不规则网络或单个二价体。根据这些标准，体内GV破裂在大多数卵母细胞中在注射HCG后20至24小时内完成。在培养中，在16至20小时之间达到类似的发育阶段。这种核变化进程的差异在核成熟开始时出现，并在整个研究期间保持不变。
