银耳酵母细胞和接合管的细胞壁组成与结构。

Cell-wall composition and structure of yeast cells and conjugation tubes of Tremella mesenterica.

作者信息

Reid I D, Bartnicki-Garcia S

出版信息

J Gen Microbiol. 1976 Sep;96(1):35-50. doi: 10.1099/00221287-96-1-35.

Abstract

Cell walls prepared from vegetative yeast cells and from hormone-induced conjugation tubes of the basidiomycete Tremella mesenterica had similar compositions. Evidence was found for 1,3-alpha-glucan (yeast 38%, tube 25%), 1,3-beta-1,6-beta-glucan (yeast 33%, tube 48%) and chitin (both less than 3%) in the walls. The walls also contained xylose (5 to 7%), mannose (6%), glucuronic acid (approx. 2%), and traces of galactose. Protein amounted to less than 2% of the wall weight. The cell capsule was very insoluble and could not be removed from the cell wall. The conjugation hormone did not appear to exert its effect on cell shape by causing gross changes in wall composition.

摘要

由营养型酵母细胞以及担子菌银耳激素诱导形成的接合管制备的细胞壁具有相似的组成。在细胞壁中发现了1,3-α-葡聚糖（酵母中占38%，接合管中占25%）、1,3-β-1,6-β-葡聚糖（酵母中占33%，接合管中占48%）和几丁质（两者均少于3%）。细胞壁还含有木糖（5%至7%）、甘露糖（6%）、葡萄糖醛酸（约2%）以及微量半乳糖。蛋白质占细胞壁重量不到2%。细胞荚膜非常不溶，无法从细胞壁上除去。接合激素似乎并非通过引起细胞壁组成的显著变化来对细胞形状发挥作用。