College of Enology, Northwest A&F University, Shaanxi, 712100, China.
Shaanxi Engineering Research Center for Viti-Viniculture, Shaanxi, 712100, China.
Sci Rep. 2017 Sep 12;7(1):11265. doi: 10.1038/s41598-017-09685-1.
Stress resistance and growth are important aspects to consider when engineering Oenococcus oeni strains for winemaking. We identified 3 previously unreported structural phenomena in the cell ontogeny of O. oeni sampled in northern China. We show that budding and binary fission (BBF) occur simultaneously in the growth process; that a novel 'pomegranate-shaped structure' (PSS) occurs mainly in the stationary and death phases; and that symbiosis and cyclical phenomena (SCP) occur throughout the various cell growth phases. These observations add to the current knowledge of the cell growth process of O. oeni. BBF, PSS, and SCP sufficiently describe the characteristics of the cellular ontogeny of O. oeni. We highlight a newly identified structure that explains the complex cell growth process. These findings will help understand the growth and development of O. oeni, supplementing the knowledge base of the established phases and providing new perspectives into its complex growth patterns.
在为葡萄酒酿造工程设计酒香酵母（Oenococcus oeni）菌株时，需要考虑其抗应激能力和生长能力。本研究在中国北方采集的酒香酵母细胞个体发育过程中发现了 3 种以前未报道的结构现象。结果表明，出芽和二分分裂（binary fission，BBF）在生长过程中同时发生；一种新型的“石榴状结构”（pomegranate-shaped structure，PSS）主要出现在静止期和死亡期；共生和循环现象（symbiosis and cyclical phenomena，SCP）贯穿细胞的各个生长阶段。这些观察结果增加了对酒香酵母细胞生长过程的认识。BBF、PSS 和 SCP 充分描述了酒香酵母细胞个体发育的特征。我们强调了一种新发现的结构，该结构解释了复杂的细胞生长过程。这些发现将有助于理解酒香酵母的生长和发育，补充已建立阶段的知识库，并为其复杂的生长模式提供新的视角。
