Mizrahi V, Andersen S J
Molecular Biology Unit, South African Institute for Medical Research, Johannesburg.
Mol Microbiol. 1998 Sep;29(6):1331-9. doi: 10.1046/j.1365-2958.1998.01038.x.
The genome sequence of Mycobacterium tuberculosis was analysed by searching for homologues of genes known to be involved in the reversal or repair of DNA damage in Escherichia coli and related organisms. Genes necessary to perform nucleotide excision repair (NER), base excision repair (BER), recombination, and SOS repair and mutagenesis were identified. In particular, all of the genes known to be directly involved in the repair of oxidative and alkylative damage are present in M. tuberculosis. In contrast, we failed to identify homologues of genes involved in mismatch repair. This finding has potentially significant implications with respect to genome stability, strain variability at repeat loci and the emergence of chromosomally encoded drug resistance mutations.
通过搜索已知参与大肠杆菌及相关生物体中DNA损伤逆转或修复的基因同源物，对结核分枝杆菌的基因组序列进行了分析。鉴定出了进行核苷酸切除修复（NER）、碱基切除修复（BER）、重组以及SOS修复和诱变所必需的基因。特别是，结核分枝杆菌中存在所有已知直接参与氧化和烷基化损伤修复的基因。相比之下，我们未能鉴定出参与错配修复的基因同源物。这一发现对于基因组稳定性、重复位点处的菌株变异性以及染色体编码的耐药性突变的出现可能具有重大意义。
