Thiel V, Siddell S G, Herold J
Institute of Virology and Immunology, University of Wuerzburg, Germany.
Adv Exp Med Biol. 1998;440:109-13. doi: 10.1007/978-1-4615-5331-1_14.
Replicons based upon the human coronavirus 229E (HCV 229E) genome were transfected into HCV 229E infected cells. We demonstrate that a synthetic RNA comprised of 646 nucloetides from the 5' end and 1465 nucloetides from the 3' end of the HCV 229E genome is replication competent. We conclude that the cis-acting elements necessary for replication are located in these 5' and 3' genomic regions. Furthermore, we inserted the intergenic region of the HCV 229E nucleocapsid protein gene into this basic construct and were able to demonstrate the transcription of "subgenomic" RNAs.
基于人冠状病毒229E（HCV 229E）基因组的复制子被转染到HCV 229E感染的细胞中。我们证明，一种由HCV 229E基因组5'端的646个核苷酸和3'端的1465个核苷酸组成的合成RNA具有复制能力。我们得出结论，复制所需的顺式作用元件位于这些5'和3'基因组区域。此外，我们将HCV 229E核衣壳蛋白基因的基因间隔区插入到这个基本构建体中，并能够证明“亚基因组”RNA的转录。
