Herold J, Thiel V, Siddell S G
Institute for Virology and Immunology, University of Würzburg, Germany.
Adv Exp Med Biol. 1998;440:265-8. doi: 10.1007/978-1-4615-5331-1_34.
A strategy to generate in vitro transcripts representing infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome is presented. PCR-DNAs were ligated in vitro, resulting in 27 kbp and 22 kbp ligation products. These DNAs can now be transcribed in vitro and the RNAs tested for infectivity and their ability to replicate.
提出了一种基于丙型肝炎病毒229E基因组生成代表感染性RNA和自主复制RNA的体外转录本的策略。PCR-DNA在体外进行连接，产生27kbp和22kbp的连接产物。现在可以对这些DNA进行体外转录，并对RNA进行感染性及其复制能力的测试。
