缺失一段保守的11个核苷酸序列对小鼠肝炎病毒RNA复制的影响。
The effect of deletion of a conserved 11 nucleotide sequence on mouse hepatitis virus RNA replication.
作者信息
Repass J F, Makino S
机构信息
Department of Microbiology, University of Texas at Austin 78712, USA.
出版信息
Adv Exp Med Biol. 1998;440:247-52. doi: 10.1007/978-1-4615-5331-1_31.
A conserved 11 nt sequence present at near the 3' end of mouse hepatitis virus (MHV) genomic RNA binds to host proteins and is important for MHV RNA replication (Yu and Leibowitz, 1995). To better understand the role of this 11 nt sequence in positive-strand MHV RNA replication, we examined whether positive-strand MHV DI RNAs from negative-strand DI RNA transcripts lacking the 11 nt sequence were synthesized in MHV-infected cells. Positive-strand DI RNAs did not accumulate efficiently, indicating that the conserved 11 nt sequence was necessary for positive-strand MHV RNA synthesis.
位于小鼠肝炎病毒（MHV）基因组RNA 3'端附近的一段保守的11个核苷酸序列可与宿主蛋白结合，对MHV RNA复制很重要（Yu和Leibowitz，1995）。为了更好地理解这段11个核苷酸序列在正链MHV RNA复制中的作用，我们检测了在缺乏该11个核苷酸序列的负链DI RNA转录本中产生的正链MHV DI RNA是否能在MHV感染的细胞中合成。正链DI RNA未能有效积累，这表明保守的11个核苷酸序列对正链MHV RNA合成是必需的。
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