人星状病毒非结构蛋白的表达与加工

Expression and processing of nonstructural proteins of the human astroviruses.

作者信息

Gibson C A, Chen J, Monroe S A, Denison M R

机构信息

Department of Microbiology and Immunology, Vanderbilt University, Nashville, Tennessee, USA.

出版信息

Adv Exp Med Biol. 1998;440:387-91. doi: 10.1007/978-1-4615-5331-1_50.

DOI:10.1007/978-1-4615-5331-1_50

PMID:9782307

Abstract

The human astroviruses (HAst) are increasingly recognized as an important cause of gastroenteritis. These viruses contain a 6.8-kb positive-sense, single-stranded RNA molecule that is infectious when transfected into permissive cells. The HAst gene 1 is composed of two open reading frames (ORFs 1a and 1b) connected by a ribosomal frameshift. Gene 1 is predicted to encode two nonstructural polyproteins (pp 1a and pp 1ab), and analysis of the HAst gene 1 sequence has resulted in predictions of a serine proteinase within the ORF1a polyprotein. However, none of the gene 1 proteins have been identified. To examine the expression and processing of the HAst2 gene 1 polyprotein, we have translated pp 1a and pp 1ab in vitro. These ongoing studies will provide the foundation for correlating gene 1 expression in vitro with proteins expressed in virus-infected cells.

摘要

人星状病毒（HAst）越来越被认为是引起肠胃炎的一个重要原因。这些病毒含有一个6.8kb的正链单链RNA分子，当转染到允许性细胞中时具有感染性。HAst基因1由两个开放阅读框（ORF1a和ORF1b）组成，它们通过核糖体移码相连。预计基因1编码两种非结构多蛋白（pp1a和pp1ab），对HAst基因1序列的分析已预测在ORF1a多蛋白中有一个丝氨酸蛋白酶。然而，基因1的蛋白质均未被鉴定出来。为了研究HAst2基因1多蛋白的表达和加工过程，我们已在体外翻译了pp1a和pp1ab。这些正在进行的研究将为将体外基因1的表达与病毒感染细胞中表达的蛋白质相关联提供基础。