受体同源物扫描在体外维持MHV - A59持续性中发挥作用。

Receptor homologue scanning functions in the maintenance of MHV-A59 persistence in vitro.

作者信息

Chen W, Yount B, Hensley L, Baric R S

机构信息

Department of Epidemiology, University of North Carolina, Chapel Hill 27599-7400, USA.

出版信息

Adv Exp Med Biol. 1998;440:743-50. doi: 10.1007/978-1-4615-5331-1_96.

DOI:10.1007/978-1-4615-5331-1_96

PMID:9782353

Abstract

Cell lines and viruses were isolated from mouse hepatitis virus (MHV-A59) persistently-infected DBT cells at different times postinfection. Cloned cell lines had cured virus infection, displayed low levels of MHVR receptor expression and were progressively more resistance to MHV infection. MHV persistence was likely maintained by epigenetic expression of the MHVR receptor in subsets of these resistant cells and by the emergence of persistent viruses characterized by high affinity MHVR receptor usage. Persistent viruses also displayed higher affinity for alternative biliary glycoprotein receptors suggesting that receptor homologue scanning functioned in the maintenance of persistence. Importantly, persistent viruses isolated after 210 days postinfection efficiently replicated in human HepG2 cells, a hepatocarcinoma cell line, suggesting that persistence promotes the interspecies transfer of MHV.

摘要

在感染后不同时间从持续感染小鼠肝炎病毒（MHV - A59）的DBT细胞中分离出细胞系和病毒。克隆的细胞系已治愈病毒感染，显示出低水平的MHVR受体表达，并且对MHV感染的抵抗力逐渐增强。MHV的持续性可能通过这些抗性细胞亚群中MHVR受体的表观遗传表达以及以高亲和力使用MHVR受体为特征的持续性病毒的出现来维持。持续性病毒对替代胆汁糖蛋白受体也表现出更高的亲和力，这表明受体同源物扫描在维持持续性中起作用。重要的是，感染后210天分离出的持续性病毒能在人肝癌细胞系HepG2细胞中有效复制，这表明持续性促进了MHV的种间转移。