Taomoto M, Nambu H, Senzaki H, Shikata N, Oishi Y, Fujii T, Miki H, Uyama M, Tsubura A
Department of Pathology, Kansai Medical University, Moriguchi, Osaka, Japan.
Graefes Arch Clin Exp Ophthalmol. 1998 Sep;236(9):688-95. doi: 10.1007/s004170050142.
The sequential retinal changes in Syrian golden hamsters induced by N-methyl-N-nitrosourea (MNU) have not been studied.
Female hamsters received a single intraperitoneal injection of 90 mg/kg MNU at 50 days of age, and the retina was examined light and electron microscopically, immunohistochemically and by the TdT-mediated dUTP-digoxigenin nick end labeling (TUNEL) method until 20 weeks after the treatment.
The retinal changes were as follows: (1) Photoreceptor apoptosis occurred 1 day after the treatment and resulted in photoreceptor loss at day 7. During the degeneration, Müller cell proliferation was conspicuous at day 5. (2) After the photoreceptor cell loss, migration of the pigment epithelial cells in all layers of the retina which were in contact with blood vessels occurred. Due to the Müller cell proliferation, gliosis was prominent at the later stage.
The MNU injection caused photoreceptor apoptosis followed by pigment epithelial cell migration around the blood vessels, accompanied by gliosis. The primary event and the course of this disease closely resemble those of retinitis pigmentosa in humans.
尚未对N-甲基-N-亚硝基脲（MNU）诱导的叙利亚金黄地鼠视网膜的连续变化进行研究。
50日龄雌性地鼠腹腔注射一次90mg/kg MNU，在治疗后20周内，通过光镜、电镜检查、免疫组织化学和TdT介导的dUTP-地高辛缺口末端标记（TUNEL）法对视网膜进行检查。
视网膜变化如下：（1）治疗后1天发生光感受器凋亡，7天时导致光感受器丧失。在退变过程中，第5天时Müller细胞增殖明显。（2）光感受器细胞丧失后，与血管接触的视网膜各层色素上皮细胞发生迁移。由于Müller细胞增殖，后期胶质增生明显。
注射MNU导致光感受器凋亡，随后色素上皮细胞围绕血管迁移，并伴有胶质增生。这种疾病的主要事件和病程与人类色素性视网膜炎极为相似。
