Kria L, Ohira A, Amemiya T
Department of Ophthalmology, Nagasaki University School of Medicine, Japan.
Graefes Arch Clin Exp Ophthalmol. 1998 Sep;236(9):702-8. doi: 10.1007/s004170050144.
In order to study growth factors in the pathogenesis and recurrence of pterygium, we grew pterygium tissues in culture and compared fibroblasts from primary and from recurrent pterygia with reference to the fibroangiogenic growth factors basic fibroblast growth factor (b-FGF), platelet-derived growth factor (PDGF), transforming growth factor beta (TGF-beta) and tumor necrosis factor alpha (TNF-alpha).
We used indirect immunohistochemical procedures against human b-FGF, PDGF, TGF-beta and TNF-alpha. As controls, we used cultured normal human conjunctival fibroblasts. A serum-free conditioned medium (CM) from confluent fibroblasts derived from primary and recurrent explants was assessed by enzyme-linked immunosorbent assay to determine the level of the above-mentioned growth factors.
Immunoreactivity of b-FGF was stronger in recurrent than in primary pterygium fibroblasts. PDGF immunolabeling was stronger in primary than in recurrent pterygium fibroblasts. TGF-beta and TNF-alpha immunolabeling was weak in both pterygia. All these growth factors were very sparse in normal conjunctival fibroblasts. Basic-FGF and TGF-beta 1 were found in the CM from both primary and recurrent pterygium, while PDGF and TNF-alpha were not detectable.
The strong immunoreactivity and the release of b-FGF in cultured fibroblasts of recurrent pterygia suggest that fibroblasts may play an important role in the recurrence of pterygium.
为了研究生长因子在翼状胬肉发病机制及复发中的作用，我们对翼状胬肉组织进行培养，并比较原发性和复发性翼状胬肉中的成纤维细胞，检测其促纤维血管生成生长因子，即碱性成纤维细胞生长因子（b-FGF）、血小板衍生生长因子（PDGF）、转化生长因子β（TGF-β）和肿瘤坏死因子α（TNF-α）。
我们采用间接免疫组织化学方法检测人b-FGF、PDGF、TGF-β和TNF-α。以培养的正常人结膜成纤维细胞作为对照。通过酶联免疫吸附测定法评估来自原发性和复发性外植体的汇合成纤维细胞的无血清条件培养基（CM），以确定上述生长因子的水平。
b-FGF在复发性翼状胬肉成纤维细胞中的免疫反应性强于原发性翼状胬肉成纤维细胞。PDGF免疫标记在原发性翼状胬肉成纤维细胞中强于复发性翼状胬肉成纤维细胞。TGF-β和TNF-α在两种翼状胬肉中的免疫标记均较弱。所有这些生长因子在正常结膜成纤维细胞中都非常稀少。在原发性和复发性翼状胬肉的CM中均发现了碱性成纤维细胞生长因子和转化生长因子β1，而未检测到血小板衍生生长因子和肿瘤坏死因子α。
复发性翼状胬肉培养的成纤维细胞中b-FGF的强免疫反应性和释放表明，成纤维细胞可能在翼状胬肉的复发中起重要作用。
