Papierniak C K, Bourey R E, Kretschmer R R, Gotoff S P, Colombetti L G
J Nucl Med. 1976 Nov;17(11):988-92.
Although the current use of 51Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium-99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte-derived chemotactic factor. The chemotactic properties of 99mTc-labeled human monocytes were preserved, and an excellent correlation between radioactive measurement and microscopic counting of migrating cells was observed.
尽管目前使用51Cr评估单核细胞趋化性已取得令人鼓舞的结果，但这种放射性核素的某些特性仍有改进空间。为此，对锝-99m作为替代标记物进行了评估。发现细胞损失了50%，但回收的细胞显示出极佳的活力和功能。使用改良的博伊登小室和淋巴细胞衍生的趋化因子来测量趋化性。99mTc标记的人单核细胞的趋化特性得以保留，并且观察到放射性测量与迁移细胞的显微镜计数之间具有极佳的相关性。
