完成嗜热栖热菌P2基因组序列测定。

Completing the sequence of the Sulfolobus solfataricus P2 genome.

作者信息

Sensen C W, Charlebois R L, Chow C, Clausen I G, Curtis B, Doolittle W F, Duguet M, Erauso G, Gaasterland T, Garrett R A, Gordon P, de Jong I H, Jeffries A C, Kozera C, Medina N, De Moors A, van der Oost J, Phan H, Ragan M A, Schenk M E, She Q, Singh R K, Tolstrup N

机构信息

National Research Council of Canada, Institute for Marine Biosciences, Halifax, NS, Canada.

出版信息

Extremophiles. 1998 Aug;2(3):305-12. doi: 10.1007/s007920050073.

DOI:10.1007/s007920050073

PMID:9783178

Abstract

The Sulfolobus solfataricus P2 genome collaborators are poised to sequence the entire 3-Mbp genome of this crenarchaeote archaeon. About 80% of the genome has been sequenced to date, with the rest of the sequence being assembled fast. In this publication we introduce the genomic sequencing and automated analysis strategy and present intial data derived from the sequence analysis. After an overview of the general sequence features, metabolic pathway studies are explained, using sugar metabolism as an example. The paper closes with an overview of repetitive elements in S. solfataricus.

摘要

嗜热栖热菌P2基因组研究团队准备对这种泉古菌的整个3兆碱基基因组进行测序。迄今为止，该基因组约80%已完成测序，其余序列的组装也进展迅速。在本出版物中，我们介绍了基因组测序和自动化分析策略，并展示了序列分析得出的初步数据。在概述了一般序列特征之后，以糖代谢为例解释了代谢途径研究。本文最后对嗜热栖热菌中的重复元件进行了概述。