Mathivanan N, Kabilan V, Murugesan K
Centre for Advanced Studies in Botany, University of Madras, India.
Can J Microbiol. 1998 Jul;44(7):646-51.
Chitinase (EC 3.2.1.14) was isolated from the culture filtrate of Fusarium chlamydosporum and purified by ion-exchange chromatography and gel filtration. The molecular mass of purified chitinase was 40 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Chitinase was optimally active at a pH of 5 and stable from pH 4 to 6 and up to 40 degrees C. Among the metals and inhibitors tested, mercuric chloride completely inhibited the enzyme activity. The activity of chitinase was high on colloidal and pure chitin. The purified chitinase inhibited the germination of uredospores of Puccinia arachidis and also lysed the walls of uredospores and germ tubes. The results from these experiments indicated that chitinase of F. chlamydosporum plays an important role in the biocontrol of groundnut rust.
几丁质酶（EC 3.2.1.14）从厚垣镰刀菌的培养滤液中分离出来，并通过离子交换色谱法和凝胶过滤法进行纯化。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳估计，纯化后的几丁质酶分子量为40 kDa。几丁质酶在pH值为5时活性最佳，在pH值4至6以及高达40摄氏度的范围内稳定。在所测试的金属和抑制剂中，氯化汞完全抑制了酶的活性。几丁质酶对胶体几丁质和纯几丁质的活性较高。纯化后的几丁质酶抑制花生柄锈菌夏孢子的萌发，还能溶解夏孢子和芽管的细胞壁。这些实验结果表明，厚垣镰刀菌的几丁质酶在花生锈病的生物防治中发挥着重要作用。
