Wimmer Z, Tykva R, Bennettová B, Vlasáková V, Elbert T
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic.
Invert Neurosci. 1997 Sep-Dec;3(2-3):193-7. doi: 10.1007/BF02480374.
A synthetic insect juvenile hormone analog (a juvenoid), ethyl N-[2-[4-[[2,2-(ethylenedioxy)cyclohexyl]methyl]phenox]ethyl]carbam ate, which has displayed high biological activity against different insect species and high stability under field conditions, was selected as a biologically active model compound for a study of a juvenile hormone analog degradation. The biologically active compound itself and its three diversely radiolabeled derivatives were applied to the flesh fly (Sarcophaga bullata) or the tsetse fly (Glossina palpalis), respectively. Monitoring of a fate of the applied juvenile hormone analog was carried out using a detection method of the radioactivity microdistribution within the whole insect body in combination with a radio high performance liquid chromatography (radio-HPLC), both of whole-body extracts made in different, but in advance scheduled, time intervals, and of extracts of insect excreta accumulated over an eight-day experiment.
一种合成昆虫保幼激素类似物（一种保幼激素类似物），N-[2-[4-[[2,2-(乙二氧基)环己基]甲基]苯氧基]乙基]氨基甲酸乙酯，已显示出对不同昆虫物种具有高生物活性且在田间条件下具有高稳定性，被选为用于研究保幼激素类似物降解的生物活性模型化合物。将生物活性化合物本身及其三种不同的放射性标记衍生物分别施用于肉蝇（Sarcophaga bullata）或采采蝇（Glossina palpalis）。通过结合放射性高效液相色谱法（radio-HPLC）对整个昆虫体内放射性微分布的检测方法，对施用的保幼激素类似物的归宿进行监测，该检测方法适用于在不同但预先设定的时间间隔制备的全身提取物以及在为期八天的实验中积累的昆虫排泄物提取物。
