Prestwich G D, Touhara K, Riddiford L M, Hammock B D
Department of Chemistry, University at Stony Brook, NY 11794-3400.
Insect Biochem Mol Biol. 1994 Sep;24(8):747-61. doi: 10.1016/0965-1748(94)90104-x.
The introduction of photoaffinity labeling into the mode of action of insect hormones and pheromones started 12 yr ago with the photoaffinity labeling of juvenile hormone binding proteins (JHBPs) from cockroaches in the laboratory of the late John K. Koeppe. Applying this technique to Manduca sexta led ultimately to a three-laboratory collaborative project that has begun to dissect the molecular basis for JH transport, metabolism, and nuclear binding and gene activation in Lepidoptera. This review provides (1) a history of the first experiments; (2) an idea of the breadth of the technique in the arthropod classes Insecta, Crustacea, and Arachnida; and (3) evidence for the depth of the technique in unearthing key details about three different types of the molecular action of JH in M. sexta.
12年前，已故的约翰·K·科佩实验室对蟑螂的保幼激素结合蛋白（JHBPs）进行光亲和标记，从而将光亲和标记引入昆虫激素和信息素的作用模式研究中。将这项技术应用于烟草天蛾最终促成了一个由三个实验室合作的项目，该项目已开始剖析鳞翅目昆虫中保幼激素运输、代谢、核结合及基因激活的分子基础。本综述提供了：（1）首次实验的历史；（2）该技术在昆虫纲、甲壳纲和蛛形纲节肢动物中的应用广度；（3）该技术在挖掘烟草天蛾中保幼激素三种不同类型分子作用关键细节方面的深度证据。
