四膜虫编码含有短内含子的非常规肌动蛋白。

Paramecium tetraurelia encodes unconventional actin containing short introns.

作者信息

Díaz-Ramos C, Villalobo E, Pérez-Romero P, Torres A

机构信息

Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Spain.

出版信息

J Eukaryot Microbiol. 1998 Sep-Oct;45(5):507-11. doi: 10.1111/j.1550-7408.1998.tb05109.x.

DOI:10.1111/j.1550-7408.1998.tb05109.x

PMID:9783451

Abstract

The polymerase chain reaction was used to amplify and clone an actin gene fragment from Paramecium tetraurelia. This DNA fragment was 1,138 bp long, more than 96% of the actin coding sequence, and contained four in-frame UAA codons and two small introns located at positions unique in the actin intron catalogue. This is the first report for the phylum Ciliophora of an actin gene containing introns. The deduced amino acid sequence of this actin fragment shared 58-77% identity with other actins. When compared with rabbit alpha-muscle actin, similarities were observed mainly in subdomains 1 and 3, whereas subdomains 2 and 4 appeared to be more divergent.

摘要

采用聚合酶链反应从四膜虫中扩增并克隆肌动蛋白基因片段。该DNA片段长1138 bp，超过肌动蛋白编码序列的96%，包含四个读框内的UAA密码子以及两个位于肌动蛋白内含子目录中独特位置的小内含子。这是纤毛门中首次报道含有内含子的肌动蛋白基因。该肌动蛋白片段推导的氨基酸序列与其他肌动蛋白的同一性为58-77%。与兔α-肌动蛋白相比，主要在亚结构域1和3中观察到相似性，而亚结构域2和4似乎差异更大。