极短的20 - 33个核苷酸的内含子是四膜虫的标准长度。
Extremely short 20-33 nucleotide introns are the standard length in Paramecium tetraurelia.
作者信息
Russell C B, Fraga D, Hinrichsen R D
机构信息
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
出版信息
Nucleic Acids Res. 1994 Apr 11;22(7):1221-5. doi: 10.1093/nar/22.7.1221.
Abstract
Paramecium tetraurelia has the shortest known introns as its standard intron length. Sequenced introns vary between 20 and 33 nucleotides in length. The intron sequences were discovered in genomic sequences coding for a variety of different proteins, including phosphatases, kinases, and low-molecular weight GTP-binding proteins. All intron sequences begin with the conserved dinucleotide GT and end with the conserved dinucleotide AG. The sequences are more AT rich than the Paramecium coding sequences. The identified sequences were confirmed as introns by sequencing several cDNA fragments. We report here analysis of the characteristics of 50 separate introns, including size, base composition, and a consensus sequence.
摘要
四膜虫（Paramecium tetraurelia）拥有已知最短的内含子作为其标准内含子长度。已测序的内含子长度在20至33个核苷酸之间变化。这些内含子序列是在编码多种不同蛋白质的基因组序列中发现的，包括磷酸酶、激酶和低分子量GTP结合蛋白。所有内含子序列均以保守的二核苷酸GT起始，并以保守的二核苷酸AG结束。这些序列比四膜虫的编码序列富含AT。通过对几个cDNA片段进行测序，确认了所鉴定的序列为内含子。我们在此报告对50个单独内含子的特征分析，包括大小、碱基组成和共有序列。
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