Tomita Y, Watanabe T, Takeuchi T, Nanbu A, Shinozaki N, Ikemi T, Fukushima K
Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Arch Oral Biol. 1998 Sep;43(9):735-40. doi: 10.1016/s0003-9969(98)00065-x.
The presence of Tween 80 in media was associated with a significant increase in three glucosyltransferases(GTFs)(I, SI and S), especially GTF-I, produced by Streptococcus mutans strain PS14, indicating that the surfactant is a major cause of the enhanced GTF production observed in cultures in M4 medium. Lecithin and Tween 20 also enhanced GTF-I production, while Triton X-100 depressed it. At a lot concentration of 0.00125%, Tween 80 enhanced markedly only GTF-I production and its effect reached maximum at a concentration of 0.0025%. Water-insoluble glucan synthesis and artificial plaque formation (in vitro sucrose-dependent colonization) by PS14 were significantly enhanced by the addition of Tween 80 at concentrations over 0.00125%. These results suggest that surfactants might vary the cariogenic potential of Strep. mutans even at low concentrations.
培养基中吐温80的存在与变形链球菌PS14菌株产生的三种葡糖基转移酶（GTF）（I、SI和S）显著增加有关，尤其是GTF-I，这表明该表面活性剂是在M4培养基培养物中观察到的GTF产量增加的主要原因。卵磷脂和吐温20也能提高GTF-I的产量，而 Triton X-100则会抑制其产量。在0.00125%的质量浓度下，吐温80仅显著提高GTF-I的产量，其效果在0.0025%的浓度下达到最大值。在浓度超过0.00125%时添加吐温80，可显著增强PS14的水不溶性葡聚糖合成和人工菌斑形成（体外蔗糖依赖性定植）。这些结果表明，即使在低浓度下，表面活性剂也可能改变变形链球菌的致龋潜力。
