Kawabata S, Torii M, Minami T, Fujiwara T, Hamada S
Department of Oral Microbiology, Osaka University Faculty of Dentistry, Japan.
J Med Microbiol. 1993 Jan;38(1):54-60. doi: 10.1099/00222615-38-1-54.
The inhibitory effect of non-ionic, anionic, cationic and ampholytic surfactants on cellular growth of Streptococcus mutans MT8148 and S. sobrinus 6715, on glucan synthesis by the purified glucosyltransferase (GTase) from these organisms, and on bacterial adherence to glass surfaces was examined in vitro. Cationic surfactants exhibited marked bactericidal activities. Anionic and ampholytic compounds were less strongly bactericidal and non-ionic surfactants produced only slight inhibition of cell growth under the conditions tested. Some non-ionic compounds had no effect on this. Glucan synthesis by GTase from mutans streptococci was inhibited by anionic and cationic surfactants. Among various GTase proteins, insoluble glucan synthesising GTases, i.e., S. mutans CA-GTase and S. sobrinus GTase-I were those most effectively inhibited by these agents. However, it was noted that whereas lower concentrations of cationic surfactants enhanced these GTase activities, higher concentrations of the surfactants were inhibitory. Non-ionic detergents stimulated soluble glucan synthesis from S. mutans CF-GTase and cationic and ampholytic surfactants enhanced or inhibited glucan synthesis depending on the concentrations of the surfactants. Sucrose-dependent cellular adherence of resting cells of mutans streptococci to glass surfaces was inhibited by the addition of surfactants that annulled the GTase activities.
在体外研究了非离子型、阴离子型、阳离子型和两性离子型表面活性剂对变形链球菌MT8148和远缘链球菌6715细胞生长、这些菌株纯化的葡糖基转移酶（GTase）合成葡聚糖以及细菌对玻璃表面黏附的抑制作用。阳离子型表面活性剂表现出显著的杀菌活性。阴离子型和两性离子型化合物的杀菌作用较弱，在测试条件下非离子型表面活性剂仅对细胞生长产生轻微抑制。一些非离子型化合物对此没有影响。变形链球菌的GTase合成葡聚糖受到阴离子型和阳离子型表面活性剂的抑制。在各种GTase蛋白中，不溶性葡聚糖合成GTase，即变形链球菌CA-GTase和远缘链球菌GTase-I，是受这些试剂抑制最有效的。然而，值得注意的是，较低浓度的阳离子型表面活性剂增强了这些GTase的活性，而较高浓度的表面活性剂则具有抑制作用。非离子型去污剂刺激变形链球菌CF-GTase合成可溶性葡聚糖，阳离子型和两性离子型表面活性剂根据其浓度增强或抑制葡聚糖合成。添加消除GTase活性的表面活性剂可抑制变形链球菌静息细胞对玻璃表面的蔗糖依赖性细胞黏附。
