Williams K, Frayne J, Hall L
Department of Biochemistry, University of Bristol, School of Medical Sciences, UK.
Mol Hum Reprod. 1998 Sep;4(9):841-8. doi: 10.1093/molehr/4.9.841.
The mammalian epididymis is the site of expression and secretion of an abundant, tissue-specific, androgen-regulated, selenium-independent, glutathione peroxidase isoenzyme (GPX5), which has been proposed to play a role in protecting the membranes of spermatozoa from the damaging effects of lipid peroxidation and/or preventing premature acrosome reaction. Using a combination of reverse transcription-polymerase chain reaction, Northern blot analysis and Western blotting, we now describe in detail the developmental expression of GPX5 transcripts and protein in the rat epididymis and characterize the association of rat GPX5 with the sperm plasma membrane.
哺乳动物的附睾是一种丰富的、组织特异性的、雄激素调节的、不依赖硒的谷胱甘肽过氧化物酶同工酶（GPX5）表达和分泌的场所，有人提出该酶在保护精子细胞膜免受脂质过氧化损伤和/或防止顶体过早反应中发挥作用。我们运用逆转录-聚合酶链反应、Northern印迹分析和蛋白质印迹法相结合的方法，详细描述了GPX5转录本和蛋白在大鼠附睾中的发育表达情况，并对大鼠GPX5与精子质膜的关联进行了表征。
