Tolbert D L, Heckroth J
Department of Anatomy and Neurobiology, Saint Louis University School of Medicine, St. Louis, Missouri, 63104, USA.
Exp Neurol. 1998 Oct;153(2):255-67. doi: 10.1006/exnr.1998.6882.
Shaker mutant rats are characterized by the adult-onset degeneration of cerebellar anterior lobe Purkinje cells and temporally correlated development of ataxia and tremor. Normal E-13 Purkinje cells were transplanted into the anterior cerebellum in adult shaker mutant rats to study donor/host interactions in an animal with adult-onset heredodegeneration. Donor Purkinje cells from extraparenchymal transplant sites migrated radially into the host molecular layer and differentiated. Donor Purkinje cell dendrites expanded to fill the host molecular layer, spinous processes were apparent, and axonal projections into the host gray and white matter were observed. Donor Purkinje cells remaining in the extraparenchymal transplant sites differentiated if they were located relatively close to the host cerebellum. Donor Purkinje cells located intraparenchymally in the host white matter or granule cell layer survived, but were stunted in their development. The orthogonal movement of donor Purkinje cells away from transplant sites in the host cerebellum was spatially restricted. The findings from this study indicate that host cerebellar cortex with adult-onset heredodegeneration of Purkinje cells supports the survival and differentiation of transplanted normal embryonic Purkinje cells.
震颤突变大鼠的特征是成年期小脑前叶浦肯野细胞退化，并伴有共济失调和震颤的时间相关性发展。将正常的胚胎第13天的浦肯野细胞移植到成年震颤突变大鼠的小脑前部，以研究成年期遗传性退化动物中供体/宿主的相互作用。来自脑外移植部位的供体浦肯野细胞呈放射状迁移到宿主分子层并分化。供体浦肯野细胞的树突扩展以填充宿主分子层，棘突明显，并且观察到轴突投射到宿主灰质和白质中。如果位于相对靠近宿主小脑的位置，留在脑外移植部位的供体浦肯野细胞会分化。位于宿主白质或颗粒细胞层内的供体浦肯野细胞存活下来，但发育受阻。供体浦肯野细胞在宿主小脑中远离移植部位的正交运动在空间上受到限制。这项研究的结果表明，具有成年期浦肯野细胞遗传性退化的宿主小脑皮质支持移植的正常胚胎浦肯野细胞的存活和分化。
