Sotelo C
Laboratoire de Neuromorphologie, Hôpital de la Salpétrière, Paris, France.
Can J Neurol Sci. 1993 May;20 Suppl 3:S43-52.
The results obtained with neuronal grafting in an animal model of heredo-degenerative ataxia (the pcd mutant mouse) have been extremely useful to unmask new aspects of neural plasticity. The grafted embryonic Purkinje cells invade the deficient molecular layer of the host by migrating radially through adult Bergmann fibers. There, they start building their dendritic trees and, by promoting the axonal sprouting of specific adult neuronal population in a timed sequence, they receive appropriate synaptic contacts, starting ten days after grafting. Twenty-one days after grafting, the grafted Purkinje cells have acquired their adult dendritic pattern and synaptic investment. Both the detailed timetable and the nature of the cellular interactions between embryonic and adult neural cells are remarkably similar to those occurring during normal development. These results raise the possibility that embryonic Purkinje cells can induce in adult neural cells a new type of plasticity, that of recreating a permissive microenvironment for the synaptic integration of the grafted neurons, leading to the anatomical restoration of the cortical circuit of the mutant cerebellum.
在遗传性退行性共济失调动物模型（pcd突变小鼠）中进行神经元移植所获得的结果，对于揭示神经可塑性的新方面极为有用。移植的胚胎浦肯野细胞通过沿成年伯格曼纤维径向迁移，侵入宿主缺失的分子层。在那里，它们开始构建树突，并且通过按时间顺序促进特定成年神经元群体的轴突发芽，在移植十天后开始接受适当的突触联系。移植21天后，移植的浦肯野细胞已获得其成年树突模式和突触联系。胚胎神经细胞与成年神经细胞之间细胞相互作用的详细时间表和性质，与正常发育过程中发生的情况非常相似。这些结果增加了一种可能性，即胚胎浦肯野细胞可以在成年神经细胞中诱导一种新型的可塑性，即为移植神经元的突触整合重新创造一个允许的微环境，从而导致突变小脑皮质回路的解剖学恢复。
