Dabrow M B, Francesco M R, McBrearty F X, Caradonna S
Lankenau Hospital and Medical Research Center, 100 Lancaster Avenue, Wynnewood, Pennsylvania 19096, USA.
Gynecol Oncol. 1998 Oct;71(1):29-37. doi: 10.1006/gyno.1998.5121.
Several growth factors have been shown to stimulate or inhibit the growth of human ovarian surface epithelial (HOSE) cells. Platelet-derived growth factor (PDGF) is likely to be released onto the ovarian surface epithelium during follicular wound repair. We undertook the evaluation of this factor and its receptor in normal and malignant ovarian cells.
The goal of this study was to evaluate the response of HOSE cells to PDGF in vitro and identify PDGF receptors on normal and malignant ovarian epithelial cells. In addition, we wanted to examine the prognostic value of the PDGF receptors in clinical specimens.
Normal HOSE cells were cultured and growth response to PDGF assayed by 3H uptake. PDGF receptor status on HOSE cells, established ovarian carcinoma cell lines, and paraffin tissue was performed by immunohistologic techniques. Data on ovarian cancer patients relapse-free survival (RFS) were abstracted from the Lankenau Hospital Tumor Registry and RFS was plotted using the Kaplan-Meier method.
HOSE cells increased 3H uptake in a dose-dependent manner in response to PDGF. HOSE cells stain positively for both alpha and beta receptors, as does the chemotherapy naive cell line A2780. The platinum-resistant CP30 cell line loses PDGF alpha staining. Of 21 ovarian cancer specimens, only 1 retained PDGF alpha receptors while 8 retained PDGF beta receptors. Those patients positive for PDGF receptor beta had a significantly longer relapse-free survival than PDGF beta receptor-negative patients.
PDGF enhances the growth of HOSE cells in vitro and may play a role in ovarian cancer development. Patients whose tumors retain PDGF receptor beta staining positivity have a prolonged relapse-free survival.
多种生长因子已被证明可刺激或抑制人卵巢表面上皮（HOSE）细胞的生长。血小板衍生生长因子（PDGF）在卵泡伤口修复过程中可能会释放到卵巢表面上皮。我们对该因子及其受体在正常和恶性卵巢细胞中的情况进行了评估。
本研究的目的是评估HOSE细胞在体外对PDGF的反应，并鉴定正常和恶性卵巢上皮细胞上的PDGF受体。此外，我们想研究PDGF受体在临床标本中的预后价值。
培养正常HOSE细胞，并通过3H摄取测定对PDGF的生长反应。通过免疫组织学技术检测HOSE细胞、已建立的卵巢癌细胞系和石蜡组织中的PDGF受体状态。从兰肯瑙医院肿瘤登记处提取卵巢癌患者无复发生存期（RFS）的数据，并使用Kaplan-Meier方法绘制RFS曲线。
HOSE细胞对PDGF的反应呈剂量依赖性增加3H摄取。HOSE细胞对α和β受体均呈阳性染色，未经化疗的细胞系A2780也是如此。铂耐药的CP30细胞系失去了PDGFα染色。在21例卵巢癌标本中，只有1例保留了PDGFα受体，而8例保留了PDGFβ受体。PDGF受体β阳性的患者无复发生存期明显长于PDGFβ受体阴性的患者。
PDGF在体外增强HOSE细胞的生长，可能在卵巢癌发展中起作用。肿瘤保留PDGF受体β染色阳性的患者无复发生存期延长。
