Bar E, Sandler N, Makayoto M, Keynan A
Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
J Invertebr Pathol. 1998 Nov;72(3):206-13. doi: 10.1006/jipa.1998.4787.
Bacillus thuringiensis israelensis delta-endotoxin genes were inserted into transposon Tn917 in plasmid pTV51Ts and cloned into the chromosome of Bacillus sphaericus 2362. Many of the transformants reacted with antibody to the 135-, 128-, 65-, and 28-kDa B.t.israelensis toxin proteins and were approximately 10 times more toxic to A. aegypti larvae than the untransformed host. Some of the transformants differed physiologically and morphologically from the wild-type B. sphaericus. The toxicity of the transformed phenotype was maintained through many transfers in the absence of selective pressure. Copyright 1998 Academic Press.
