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Identification of the nuclear factor HMG2 as an activator for DFF nuclease activity.

作者信息

Toh S Y, Wang X, Li P

机构信息

Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore, 117609, Singapore.

出版信息

Biochem Biophys Res Commun. 1998 Sep 29;250(3):598-601. doi: 10.1006/bbrc.1998.9369.

DOI:10.1006/bbrc.1998.9369
PMID:9784391
Abstract

DNA fragmentation during apoptosis is mediated by a heterodimeric protein complex, DFF45/ICAD and DFF40/CAD/CPAN. Purified DFF40 alone possesses intrinsic nuclease activity that is inhibited by its association with DFF45. The proteolytic cleavage of DFF45 by caspase-3 frees the DFF40 subunit to function as a nuclease. In the course of identifying factors that stimulate DFF activity, we have isolated a nuclear factor and identified it as the high mobility group protein 2 (HMG2). We found that bacterially expressed HMG2 is able to enhance the nuclease activity of DFF. As HMG2 has DNA bending activity (6), our data suggest that HMG proteins may augment DNA fragmentation during apoptosis through changes in chromosome structure.

摘要

相似文献

1
Identification of the nuclear factor HMG2 as an activator for DFF nuclease activity.
Biochem Biophys Res Commun. 1998 Sep 29;250(3):598-601. doi: 10.1006/bbrc.1998.9369.
2
Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD.凋亡核酸酶DFF40/CAD中调控域和催化域的鉴定
J Biol Chem. 1999 Jan 1;274(1):270-4. doi: 10.1074/jbc.274.1.270.
3
Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state.DNA片段化因子（DFF）与DNA的相互作用揭示了一种前所未有的核酸酶抑制机制，并表明DFF可以在与DNA结合的状态下被激活。
J Biol Chem. 2005 Feb 18;280(7):6005-15. doi: 10.1074/jbc.M413035200. Epub 2004 Nov 30.
4
Activation of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease). Oligomerization and direct interaction with histone H1.凋亡核酸内切酶DFF40（半胱天冬酶激活的脱氧核糖核酸酶或核酸酶）的激活。寡聚化以及与组蛋白H1的直接相互作用。
J Biol Chem. 1999 May 14;274(20):13836-40. doi: 10.1074/jbc.274.20.13836.
5
Oligomerization state of the DNA fragmentation factor in normal and apoptotic cells.正常细胞和凋亡细胞中DNA片段化因子的寡聚化状态。
J Biol Chem. 2005 Dec 2;280(48):40216-25. doi: 10.1074/jbc.M502220200. Epub 2005 Oct 3.
6
Subunit structures and stoichiometries of human DNA fragmentation factor proteins before and after induction of apoptosis.
J Biol Chem. 2003 Jul 18;278(29):26915-22. doi: 10.1074/jbc.M303807200. Epub 2003 May 14.
7
Multiple domains of DFF45 bind synergistically to DFF40: roles of caspase cleavage and sequestration of activator domain of DFF40.DFF45的多个结构域与DFF40协同结合：半胱天冬酶切割和隔离DFF40激活结构域的作用
Biochem Biophys Res Commun. 1999 Oct 14;264(1):181-5. doi: 10.1006/bbrc.1999.1498.
8
The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis.DNA片段化因子的40 kDa亚基在细胞凋亡过程中诱导DNA片段化和染色质浓缩。
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8461-6. doi: 10.1073/pnas.95.15.8461.
9
Caspase-3 is the primary activator of apoptotic DNA fragmentation via DNA fragmentation factor-45/inhibitor of caspase-activated DNase inactivation.半胱天冬酶-3是通过DNA片段化因子45/半胱天冬酶激活的脱氧核糖核酸酶失活抑制剂实现凋亡性DNA片段化的主要激活剂。
J Biol Chem. 1999 Oct 22;274(43):30651-6. doi: 10.1074/jbc.274.43.30651.
10
Solution structure of DFF40 and DFF45 N-terminal domain complex and mutual chaperone activity of DFF40 and DFF45.DFF40与DFF45 N端结构域复合物的溶液结构以及DFF40和DFF45的相互伴侣活性
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6051-5. doi: 10.1073/pnas.111145098.

引用本文的文献

1
Non-endometrioid and high-grade endometrioid endometrial cancers show DNA fragmentation factor 40 (DFF40) and B-cell lymphoma 2 protein (BCL2) underexpression, which predicts disease-free and overall survival, but not DNA fragmentation factor 45 (DFF45) underexpression.非子宫内膜样和高级别子宫内膜样癌表现出 DNA 碎片因子 40（DFF40）和 B 细胞淋巴瘤 2 蛋白（BCL2）表达不足，这预测了无病生存和总生存，但不能预测 DNA 碎片因子 45（DFF45）表达不足。
BMC Cancer. 2018 Apr 13;18(1):418. doi: 10.1186/s12885-018-4333-6.
2
Contrasting nuclear dynamics of the caspase-activated DNase (CAD) in dividing and apoptotic cells.分裂细胞和凋亡细胞中半胱天冬酶激活的脱氧核糖核酸酶（CAD）的核动力学对比
J Cell Biol. 2004 Dec 6;167(5):851-62. doi: 10.1083/jcb.200404105. Epub 2004 Nov 29.
3
HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T-lymphocyte protease granzyme A.HMG2与核小体组装蛋白SET相互作用，并且是细胞毒性T淋巴细胞蛋白酶颗粒酶A的作用靶点。
Mol Cell Biol. 2002 Apr;22(8):2810-20. doi: 10.1128/MCB.22.8.2810-2820.2002.
4
Involvement of conserved histidine, lysine and tyrosine residues in the mechanism of DNA cleavage by the caspase-3 activated DNase CAD.半胱天冬酶-3激活的脱氧核糖核酸酶CAD切割DNA的机制中保守组氨酸、赖氨酸和酪氨酸残基的参与。
Nucleic Acids Res. 2002 Mar 15;30(6):1325-32. doi: 10.1093/nar/30.6.1325.
5
Identification of functionally relevant histidine residues in the apoptotic nuclease CAD.凋亡核酸酶CAD中功能相关组氨酸残基的鉴定
Nucleic Acids Res. 2001 Oct 1;29(19):3901-9. doi: 10.1093/nar/29.19.3901.
6
Solution structure of DFF40 and DFF45 N-terminal domain complex and mutual chaperone activity of DFF40 and DFF45.DFF40与DFF45 N端结构域复合物的溶液结构以及DFF40和DFF45的相互伴侣活性
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6051-5. doi: 10.1073/pnas.111145098.