Lundström A, Holmbom J, Lindqvist C, Nordström T
Department of Biochemistry and Pharmacy, Abo Akademi University, BioCity, Turku, Finland.
Biochem Biophys Res Commun. 1998 Sep 29;250(3):735-40. doi: 10.1006/bbrc.1998.9389.
Molecular mechanisms involved in the rapid attachment of the human breast cancer cell line MDA-MD-231 to cortical bone matrix were studied. The attachment of MDA-MD-231 cells to cortical bone disks could be blocked by 75% when cells were pretreated with a monoclonal antibody to the beta1-subunit of the integrin family. Monoclonal antibodies against the alpha2, alpha3, and alpha5 integrin subunits inhibited the attachment by 76, 26, and 8 % respectively. Collagenase type I and collagen type I antibody blocked the cell attachment by 45 and 50 % whereas pretreatment of the cells with soluble collagen type I blocked the attachment by 85 %. Our study with a panel of cancer cell lines further showed a close correlation between alpha2 beta1 and alpha3 beta1 integrin receptor expression and the capability to attach to cortical bone. These receptors appear to be the key receptors utilized by cancer cells for the initial attachment to cortical bone, and this could facilitate the localization of alpha2 beta1 and alpha3 beta1 expressing cancer cells to the skeleton.
研究了人乳腺癌细胞系MDA-MD-231快速附着于皮质骨基质的分子机制。当用针对整合素家族β1亚基的单克隆抗体预处理细胞时，MDA-MD-231细胞与皮质骨盘的附着可被阻断75%。针对α2、α3和α5整合素亚基的单克隆抗体分别抑制附着76%、26%和8%。I型胶原酶和I型胶原抗体分别阻断细胞附着45%和50%，而用可溶性I型胶原预处理细胞则阻断附着85%。我们对一组癌细胞系的研究进一步表明，α2β1和α3β1整合素受体表达与附着于皮质骨的能力之间存在密切相关性。这些受体似乎是癌细胞用于最初附着于皮质骨的关键受体，这可能有助于表达α2β1和α3β1的癌细胞在骨骼中的定位。
