来自黄化水稻幼苗的蔗糖合酶同工酶的纯化与特性分析
Purification and characterization of sucrose synthase isozymes from etiolated rice seedlings.
作者信息
Huang D Y, Wang A Y
机构信息
Department of Agricultural Chemistry, National Taiwan University, Taipei.
出版信息
Biochem Mol Biol Int. 1998 Sep;46(1):107-13. doi: 10.1080/15216549800203612.
Presence of homo- and hetero-tetrameric rice sucrose synthase (RSS) isoforms in etiolated rice seedlings was demonstrated by immunoprecipitation using monospecific antibodies. Three RSS isozymes with various pI values were purified by ammonium sulfate fractionation, Sepharose CL-6B gel filtration, DEAE-Sephacel and Mono-Q ion exchange chromatographies. They were characterized as heterotetramers composed of RSS1 and RSS2 subunits. All of them used UDP as the best substrate. The presence of divalent metal ions increased the activities of synthesis but inhibited the cleavage of sucrose.
通过使用单特异性抗体进行免疫沉淀，证明了黄化水稻幼苗中存在同源和异源四聚体水稻蔗糖合酶（RSS）同工型。通过硫酸铵分级分离、Sepharose CL-6B凝胶过滤、DEAE-Sephacel和Mono-Q离子交换色谱法纯化了三种具有不同pI值的RSS同工酶。它们被表征为由RSS1和RSS2亚基组成的异源四聚体。它们都将UDP用作最佳底物。二价金属离子的存在增加了合成活性，但抑制了蔗糖的裂解。
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