Chróścicka Anna, Komorowski S, Maleszewski Marek
Department of Embryology, Institute of Zoology, Warsaw University, Poland.
Mol Reprod Dev. 2004 Jul;68(3):308-12. doi: 10.1002/mrd.20081.
To track the lineage of both blastomeres of 2-cell embryos during mouse preimplantation development, each cell was injected with dextran solutions conjugated with different fluorochromes. The fate of the progeny of the first two blastomeres was followed with confocal microscopy during cleavage and during the formation of the blastocyst. We observed that in most of cleaving embryos the cells derived from the two first blastomeres intermingled in both the trophectoderm and the inner cell mass (ICM) and did not form two discrete groups. We conclude that embryonic parts of blastocysts contain descendants of both blastomeres of 2-cell embryo.
为追踪小鼠植入前发育过程中2细胞胚胎两个卵裂球的谱系，向每个细胞注射与不同荧光染料偶联的葡聚糖溶液。在卵裂期和囊胚形成过程中，利用共聚焦显微镜追踪最初两个卵裂球后代的命运。我们观察到，在大多数正在卵裂的胚胎中，源自最初两个卵裂球的细胞在滋养外胚层和内细胞团（ICM）中相互混合，并未形成两个离散的群体。我们得出结论，囊胚的胚胎部分包含2细胞胚胎两个卵裂球的后代。
