冷冻保存的人类精液在辅助生殖中的最佳利用：精子活力和生存能力的恢复与维持。

Optimal utilization of cryopreserved human semen for assisted reproduction: recovery and maintenance of sperm motility and viability.

作者信息

Polcz T E, Stronk J, Xiong C, Jones E E, Olive D L, Huszar G

机构信息

Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.

出版信息

J Assist Reprod Genet. 1998 Sep;15(8):504-12. doi: 10.1023/a:1022586505089.

DOI:10.1023/a:1022586505089

PMID:9785198

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455048/

Abstract

PURPOSE

Our purpose was to evaluate sperm motility and viability and the maintenance of these parameters in already cryopreserved semen samples following repeated freezing/thawing cycles.

METHODS

Human spermatozoa were subjected to five cycles of cryopreservation/thawing. Recovery of sperm motility and viability and the proportion of viable nonmotile sperm were determined up to 6 hr after thaw.

RESULTS

Sperm motilities (prefreeze motility, 70.1%; n = 9 samples) after each of five freeze/thaw cycles were 24.4, 8.0, 3.5, 1.5 and 1.8%. The recovery of sperm viability was higher than that of motility after each cycle: 39.1, 25.3, 22.6, 17.8, and 16.5%. Recoveries of motility and viability were improved if the thawed samples were left in the original cryopreservation medium prior to refreezing vs. if a washing/ resuspension step was included. The recovery of sperm motility in the first thawing cycle was indicative of the expected motile sperm recovery in the second thawing cycle.

CONCLUSIONS

Cryopreserved semen that is intended to be reused in future assisted reproduction treatments should be thawed only once and aliquoted in the original freezing medium before refreezing. The recovery of sperm motility and viability in the second thawing cycle, thus the applicability of the sample in conventional in vitro fertilization or intracytoplasmic sperm injection may be anticipated in > 90% of the samples. In view of intracytoplasmic sperm injection it is important that sperm viability is maintained better than motility; after the first, second, and third thawing cycles the ratios of motile:nonmotile viable sperm were 1:1, 1:4, and 1:7, respectively.

摘要

目的

我们的目的是评估精子活力和生存能力，以及在已经冷冻保存的精液样本经过反复冻融循环后这些参数的维持情况。

方法

人类精子经历五个冻融循环。在解冻后长达6小时内测定精子活力和生存能力的恢复情况以及存活但无运动能力精子的比例。

结果

五个冻融循环中每个循环后的精子活力（冻前活力为70.1%；n = 9个样本）分别为24.4%、8.0%、3.5%、1.5%和1.8%。每个循环后精子生存能力的恢复高于活力的恢复：分别为39.1%、25.3%、22.6%、17.8%和16.5%。与包含洗涤/重悬步骤相比，如果解冻后的样本在重新冷冻前留在原始冷冻保存介质中，活力和生存能力的恢复会得到改善。第一个解冻循环中精子活力的恢复预示着第二个解冻循环中预期的有运动能力精子的恢复情况。

结论

打算在未来辅助生殖治疗中重复使用的冷冻保存精液应仅解冻一次，并在重新冷冻前在原始冷冻介质中进行分装。在第二个解冻循环中精子活力和生存能力的恢复情况，以及因此样本在常规体外受精或胞浆内单精子注射中的适用性，在超过90%的样本中是可以预期的。鉴于胞浆内单精子注射，保持精子生存能力优于活力很重要；在第一、第二和第三个解冻循环后，有运动能力的存活精子与无运动能力的存活精子的比例分别为1:1、1:4和1:7。