Habrun B, Bilić V, Humski A
Croatian Veterinary Institute, Department of Bacteriology, Zagreb, Croatia.
Prev Vet Med. 1998 Sep 1;36(3):179-86. doi: 10.1016/s0167-5877(98)00088-9.
The aim of the study was to compare the results of ELISA and 2-META assays in serological diagnosis of swine pleuropneumonia in breeding pigs of different ages. A total of 874 sera were examined for the presence of antibodies against Actinobacillus (A.) pleuropneumoniae serotype 2. The sera had been taken from 437 gilts (aged approximately 6 months), 334 sows (> 2 years of age) and 104 boars (1-2 years). Sixty-nine sera of the gilts and sows, and 46 sera of the boars were also examined for the presence of antibodies against A. pleuropneumoniae serotype 4-7. Sera from the youngest age group (gilts) showed the highest agreement between the results obtained by the two assays, with a kappa value of 0.633 and 0.437 for detection of antibodies against A. pleuropneumoniae serotype 2 and 4-7, respectively. The lowest agreement of the two assays was recorded in the oldest age group (sows), with a kappa value of 0.128 and 0.199 for detection of antibodies against A. pleuropneumoniae serotype 2 and 4-7, respectively. Boars were older than gilts and younger than sows. In this group of animals, agreement between the results of the two assays was inferior to that found in gilts and superior to that recorded in sows.
本研究的目的是比较酶联免疫吸附测定（ELISA）和2-巯基乙醇（2-META）测定法在不同年龄种猪血清学诊断猪胸膜肺炎中的结果。共检测了874份血清中抗胸膜肺炎放线杆菌2型抗体的存在情况。这些血清取自437头后备母猪（约6月龄）、334头母猪（2岁以上）和104头公猪（1 - 2岁）。还检测了69份后备母猪和母猪的血清以及46份公猪的血清中抗胸膜肺炎放线杆菌4 - 7型抗体的存在情况。最年轻年龄组（后备母猪）的血清在两种检测方法所得结果之间显示出最高的一致性，检测抗胸膜肺炎放线杆菌2型和4 - 7型抗体的kappa值分别为0.633和0.437。两种检测方法一致性最低的是最年长年龄组（母猪），检测抗胸膜肺炎放线杆菌2型和4 - 7型抗体的kappa值分别为0.128和0.199。公猪年龄大于后备母猪且小于母猪。在这组动物中，两种检测方法结果之间的一致性低于后备母猪组且高于母猪组。
