Bougrine S I, Fihri O F, Fehri M M
Département de Microbiologie Immunologie Maladies Contagieuses, Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco.
Arch Virol Suppl. 1998;14:329-36. doi: 10.1007/978-3-7091-6823-3_29.
A Western immunoblotting procedure has been developed for the detection of African horse sickness virus (AHSV) protein-specific antibody responses. This assay readily identifies antibodies specific for at least 4 distinct, AHSV proteins, including VP5, NS1, NS2 and NS3/NS3a. By using the AHSV non-structural proteins as 'markers', the Western blotting procedure could be employed to provide a reliable means of discriminating between animals vaccinated with a purified, inactivated AHSV vaccine and those either naturally infected or vaccinated with a live, attenuated AHSV vaccine.
已开发出一种蛋白质免疫印迹法用于检测非洲马瘟病毒（AHSV）的蛋白特异性抗体反应。该检测方法能轻易识别至少4种不同的AHSV蛋白的特异性抗体，包括VP5、NS1、NS2和NS3/NS3a。通过使用AHSV非结构蛋白作为“标志物”，蛋白质免疫印迹法可用于提供一种可靠的方法，以区分接种纯化灭活AHSV疫苗的动物与自然感染或接种减毒活AHSV疫苗的动物。
