Bègue-Kirn C, Krebsbach P H, Bartlett J D, Butler W T
Department of Basic Sciences, Dental Branch, University of Texas Houston Health Science Center 77030, USA.
Eur J Oral Sci. 1998 Oct;106(5):963-70. doi: 10.1046/j.0909-8836.1998.eos106510.x.
Dentin sialophosphoprotein [designated DSPP and cleaved into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP)], enamelysin and ameloblastin are each expressed in unique fashions during tooth development. It is possible that these components participate in cell differentiation and the conversion of unmineralized matrix into mineralized structures. In order to delineate the timing and the positioning of these three molecules in a physiological context, we compared their expression profiles by performing in situ hybridization experiments on consecutive sections in developing mouse tissues. Hybridization signals were uniquely detected for DSPP mRNA in odontoblasts and preameloblasts, for enamelysin mRNA in odontoblasts and in the facing ameloblast layer, and for ameloblastin mRNA in preodontoblasts, polarizing odontoblasts and ameloblasts. Immunohistochemistry showed that DSP and ameloblastin transcripts were translated into proteins that were deposited at the apical pole of the differentiated cells (odontoblasts and ameloblasts, respectively). The interrelated expression profiles found for these tooth-specific molecules illustrate the importance of a specific molecular network to initiate highly regulated processes such as cytodifferentiation and the subsequent mineralization.
牙本质涎磷蛋白（命名为DSPP，可裂解为牙本质涎蛋白（DSP）和牙本质磷蛋白（DPP））、釉质溶解蛋白和成釉蛋白在牙齿发育过程中均以独特的方式表达。这些成分可能参与细胞分化以及未矿化基质向矿化结构的转化。为了在生理背景下确定这三种分子的表达时间和定位，我们通过对发育中小鼠组织的连续切片进行原位杂交实验，比较了它们的表达谱。在成牙本质细胞和前成釉细胞中独特地检测到DSPP mRNA的杂交信号，在成牙本质细胞和相对的成釉细胞层中检测到釉质溶解蛋白mRNA的杂交信号，在成牙本质细胞前体细胞、极化的成牙本质细胞和成釉细胞中检测到成釉蛋白mRNA的杂交信号。免疫组织化学显示，DSP和成釉蛋白转录本被翻译成蛋白质，并分别沉积在分化细胞（成牙本质细胞和成釉细胞）的顶端极。这些牙齿特异性分子的相关表达谱说明了特定分子网络对启动细胞分化和随后矿化等高度调控过程的重要性。
