Bègue-Kirn C, Ruch J V, Ridall A L, Butler W T
Department of Basic Sciences, University of Texas Health Science Center, Dental Branch, Houston 77030, USA.
Eur J Oral Sci. 1998 Jan;106 Suppl 1:254-9. doi: 10.1111/j.1600-0722.1998.tb02184.x.
Dentin sialoprotein (DSP) and dentin phosphoproteins (DPP) are uniquely expressed by differentiating and fully differentiated mature odontoblasts. It is likely that DSP and DPP actively participate in the conversion of predentin to dentin. To compare the expression patterns of DPP and DSP, we constructed mouse cDNA probes. Northern analyses confirmed their specific expressions in tooth germ-derived RNA and showed that the probes reacted with similar or identical multiple transcripts. In situ hybridization indicated that DSP and DPP transcripts were uniquely detected and codistributed in developing mouse odontoblasts and preameloblasts. These data, as well as the adjacent positioning of the DSP and DPP coding sequences, suggest that common regulatory mechanisms control DSP and DPP expressions. Dental papillae cultures, in which odontoblast differentiation was experimentally induced with TGFbeta1 combined with heparin, were used to show that the two molecules are also coexpressed under in vitro conditions.
牙本质涎蛋白（DSP）和牙本质磷蛋白（DPP）由分化中的及完全分化的成熟成牙本质细胞特异性表达。DSP和DPP很可能积极参与前期牙本质向牙本质的转化。为比较DPP和DSP的表达模式，我们构建了小鼠cDNA探针。Northern分析证实了它们在牙胚来源的RNA中的特异性表达，并表明探针与相似或相同的多个转录本发生反应。原位杂交表明，在发育中的小鼠成牙本质细胞和前成釉细胞中能特异性检测到DSP和DPP转录本且它们共分布。这些数据，以及DSP和DPP编码序列的相邻定位，提示共同的调控机制控制DSP和DPP的表达。利用牙乳头培养物（其中用转化生长因子β1联合肝素实验性诱导成牙本质细胞分化）来表明这两种分子在体外条件下也共表达。
